1R5P

Crystal Structure Analysis of KaiB from PCC7120

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	200
Detector technology	CCD
Collection date	2003-02-21
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	104.755, 36.387, 54.140
Unit cell angles	90.00, 98.83, 90.00

Refinement procedure

Resolution	25.880 - 2.200
Rwork	0.212
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.440
High resolution limit [Å]	2.200	2.200
Number of reflections	10484
Completeness [%]	93.4	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K