1R5O
crystal structure analysis of sup35 complexed with GMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9724 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 82.524, 82.524, 168.076 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.26836 |
Rwork | 0.266 |
R-free | 0.31959 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r5b |
RMSD bond length | 0.012 |
RMSD bond angle | 1.377 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.140 | 0.140 |
Number of reflections | 11894 | |
<I/σ(I)> | 5.2 | 0.7 |
Completeness [%] | 98.3 | 98.3 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | HEPES, Ethylene Glycol, PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |