1R5N

Crystal Structure Analysis of sup35 complexed with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-10
Detector	MARRESEARCH
Wavelength(s)	0.9724
Spacegroup name	P 43 21 2
Unit cell lengths	82.763, 82.763, 169.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.25719
Rwork	0.256
R-free	0.28415
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r5b
RMSD bond length	0.011
RMSD bond angle	1.252
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.075	0.075
Number of reflections	16838
<I/σ(I)>	9	0.7
Completeness [%]	100.0	100
Redundancy		7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	HEPES, Ethylene Glycol, PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K