1R5B
Crystal structure analysis of sup35
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-12 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9787, 0.9724, 0.9791 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 83.796, 83.796, 165.937 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.350 |
R-factor | 0.256 |
Rwork | 0.255 |
R-free | 0.28400 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.290 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.410 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.047 | 0.375 |
Number of reflections | 24790 | |
<I/σ(I)> | 11.2 | 2 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 6.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | HEPES, Ethylene Glycol, PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |