1R53
Crystal structure of the bifunctional chorismate synthase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 57.516, 74.664, 155.032 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.300 * - 2.200 |
R-factor | 0.17626 |
Rwork | 0.174 |
R-free | 0.22500 * |
Structure solution method | SAD |
RMSD bond length | 0.034 |
RMSD bond angle | 1.720 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.000 * | |
High resolution limit [Å] | 2.200 | |
Rmerge | 0.050 * | 0.150 * |
Total number of observations | 63849 * | |
Number of reflections | 17154 * | |
<I/σ(I)> | 10.9 | |
Completeness [%] | 96.4 * | 77.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | Na-Citrate, EDTA, DDT, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | sodium cirtate | 0.7 (M) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
4 | 1 | reservoir | EDTA | 10 (mM) | |
5 | 1 | reservoir | dithiothreitol | 10 (mM) |