Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 81.300, 96.300, 105.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.750 |
| R-factor | 0.183 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1UOX |
| RMSD bond length | 0.029 |
| RMSD bond angle | 0.074 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 38367 | |
| <I/σ(I)> | 19.8 | 4.8 |
| Completeness [%] | 92.1 | 79.6 |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 8.5MG/ML PROTEIN, 0.2MG/ML 8-AZAXANTHIN 5-7% W/V PEG8000, 100MM TRIS/HCL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
