1R2O
d(GCATGCT) + Ni2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8126 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 24.807, 24.807, 92.081 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.710 - 2.380 |
| R-factor | 0.22558 |
| Rwork | 0.224 |
| R-free | 0.24596 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.467 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.710 | 2.510 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.082 | 0.216 |
| Number of reflections | 857 | |
| <I/σ(I)> | 9.9 | 4 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 13.47 | 17.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 290 | sodium cacodylate, nickel dichloride, potassium chloride, MPD, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 1 | nickel dichloride | ||
| 3 | 1 | 1 | potassium chloride | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | sodium cacodylate | ||
| 7 | 1 | 2 | nickel dichloride | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
