1R2M
Atomic resolution structure of the HFBII hydrophobin: a self-assembling amphiphile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8126 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.657, 46.310, 34.593 |
| Unit cell angles | 90.00, 112.16, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.000 |
| R-factor | 0.13789 |
| Rwork | 0.137 |
| R-free | 0.15500 * |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.394 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.063 * | 0.235 * |
| Total number of observations | 214050 * | |
| Number of reflections | 61978 | |
| <I/σ(I)> | 7.6 | 3.2 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 2000, LiSO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 | 25 (%) | |
| 2 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
| 5 | 1 | drop | protein | 8 (mg/ml) |
