1R2F

RIBONUCLEOTIDE REDUCTASE R2F PROTEIN FROM SALMONELLA TYPHIMURIUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	58.490, 71.740, 96.070
Unit cell angles	90.00, 95.01, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.226
Rwork	0.226
R-free	0.24700
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.173
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.069 *	0.230 *
Number of reflections	45114
<I/σ(I)>	8.6
Completeness [%]	96.6	93.8 *
Redundancy	3.4	2.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	15 *	pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	50 (mM)
2	1	drop	dithiothreitol	10 (mM)
3	1	drop	protein	10-30 (mg/ml)
4	1	reservoir	PEG8000	15-25 (%)
5	1	reservoir	Tris
6	1	reservoir		0.7 (M)