1R2C

PHOTOSYNTHETIC REACTION CENTER BLASTOCHLORIS VIRIDIS (ATCC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	293
Detector technology	CCD
Collection date	2002-08-16
Detector	ADSC QUANTUM 4
Spacegroup name	P 43 21 2
Unit cell lengths	223.500, 223.500, 112.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.830 - 2.860
R-factor	0.202
Rwork	0.202
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3PRC (CHAINS ONLY)
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.830	3.040
High resolution limit [Å]	2.862	2.860
Rmerge	0.085	0.447
Number of reflections	64248
<I/σ(I)>	7.6	1.7
Completeness [%]	97.9	97.2
Redundancy	7.1	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8	291	ammonium sulfate, sodium phosphate, LDAO, 1,2,3-heptanetriol, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 291K, pH 6.80