1R29
Crystal Structure of the B-Cell Lymphoma 6 (BCL6) BTB Domain to 1.3 Angstrom
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97947, 0.97925, 0.95742, 0.8980 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 30.606, 71.849, 55.414 |
| Unit cell angles | 90.00, 105.91, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.300 |
| R-factor | 0.128 |
| Rwork | 0.128 |
| R-free | 0.17300 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.530 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.051 * | 0.302 * |
| Number of reflections | 27157 | |
| <I/σ(I)> | 24.4 | 4.3 |
| Completeness [%] | 95.7 | 93.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | sodium formate, sodium acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | sodium formate | 0.18 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.8 |
