1R29

Crystal Structure of the B-Cell Lymphoma 6 (BCL6) BTB Domain to 1.3 Angstrom

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97947, 0.97925, 0.95742, 0.8980
Spacegroup name	C 1 2 1
Unit cell lengths	30.606, 71.849, 55.414
Unit cell angles	90.00, 105.91, 90.00

Refinement procedure

Resolution	30.000 - 1.300
R-factor	0.128
Rwork	0.128
R-free	0.17300
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.530
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.350
High resolution limit [Å]	1.300	1.300
Rmerge	0.051 *	0.302 *
Number of reflections	27157
<I/σ(I)>	24.4	4.3
Completeness [%]	95.7	93.7
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	298	sodium formate, sodium acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	sodium formate	0.18 (M)
3	1	reservoir	sodium acetate	0.1 (M)	pH4.8