1R1N
Tri-nuclear oxo-iron clusters in the ferric binding protein from N. gonorrhoeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 146.500, 146.500, 114.969 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.740 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o7t |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.820 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.117 | 0.470 |
| Number of reflections | 280085 | |
| <I/σ(I)> | 7.1 | 2.1 |
| Completeness [%] | 99.0 | 87.1 |
| Redundancy | 8.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | PEG 1450, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
