1R1J

STRUCTURAL ANALYSIS OF NEPRILYSIN WITH VARIOUS SPECIFIC AND POTENT INHIBITORS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-08-15
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	107.458, 107.458, 111.997
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.350
R-factor	0.22482
Rwork	0.222
R-free	0.28325
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.718
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.350	2.350
Rmerge	0.090	0.480
Number of reflections	31584
<I/σ(I)>	14.7	3.1
Completeness [%]	99.8	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1