1R14
Carbohydrate recognition and neck domains of surfactant protein A (Sp-A) containing samarium
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.771, 1.8450, 1.8456 |
Spacegroup name | P 63 |
Unit cell lengths | 97.680, 97.680, 44.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.24700 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.154 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.073 | 0.338 |
Total number of observations | 101169 * | |
Number of reflections | 8556 | |
<I/σ(I)> | 27.5 | 4.9 |
Completeness [%] | 98.9 | 91.5 |
Redundancy | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 17 * | LiSO4, CaCl2, glycerol, trimannose, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 5 (mM) | |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | protein | 3 (mg/ml) | |
4 | 1 | reservoir | 1.6 (M) | ||
5 | 1 | reservoir | 10 (mM) | ||
6 | 1 | reservoir | glycerol | 7 (%(w/v)) | |
7 | 1 | reservoir | MES | 100 (mM) | pH6.5 |