1R11
Structure Determination of the Dimeric Endonuclease in a Pseudo-face-centerd P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-3 |
Synchrotron site | SSRL |
Beamline | BL9-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-11-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.412, 74.169, 84.855 |
Unit cell angles | 90.00, 106.13, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.000* |
R-factor | 0.2369 |
Rwork | 0.231 |
R-free | 0.22900 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.163 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | |
High resolution limit [Å] | 2.000 * | 2.000 * |
Rmerge | 0.088 * | 0.352 * |
Number of reflections | 77465 * | |
Completeness [%] | 90.8 * | 76.5 * |
Redundancy | 3.89 * | 2.46 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.6 | 100 mM Na-cacodylic acid pH 6.5 - 6.8, 20 mM (NH4)2SO4, 300-400 mM NaCH2COOH, pH 6.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 60 (mg/ml) | |
2 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5-6.8 |
3 | 1 | reservoir | ammonium sulfate | 20 (mM) | |
4 | 1 | reservoir | sodium acetate | 300-400 (mM) |