1R0Z
Phosphorylated Cystic fibrosis transmembrane conductance regulator (CFTR) nucleotide-binding domain one (NBD1) with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9793 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 171.330, 171.330, 109.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.000 - 2.350 |
R-factor | 0.225 |
Rwork | 0.221 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.090 * | 0.460 * |
Number of reflections | 67494 | |
Completeness [%] | 97.6 | 92.4 |
Redundancy | 4.6 * | 2.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4, 20 * | sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-10 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 3.5-4.0 (M) |