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1R0W

Cystic fibrosis transmembrane conductance regulator (CFTR) nucleotide-binding domain one (NBD1) apo

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 5.0.1
Synchrotron siteALS
Beamline5.0.1
Temperature [K]100
Detector technologyCCD
Collection date2003-05-16
DetectorMARRESEARCH
Wavelength(s)1.000
Spacegroup nameP 4 21 2
Unit cell lengths172.247, 172.247, 110.233
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution38.900 - 2.200
R-factor0.231
Rwork0.212
R-free0.26200
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.021
RMSD bond angle2.000
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareEPMR
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]38.9002.280
High resolution limit [Å]2.2002.200
Rmerge0.076

*

0.440

*

Number of reflections70947
Completeness [%]93.295.1
Redundancy5.1

*

4.1

*

Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.54, 20

*

sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein5-10 (mg/ml)
21reservoirsodium acetate3.5-4.0 (M)

221716

PDB entries from 2024-06-26

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