1R0U
Crystal structure of ywiB protein from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-01 |
| Detector | SBC-2 |
| Wavelength(s) | 1.03320, 0.97935, 0.97945, 0.96422 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.428, 48.428, 136.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.170 - 1.750 |
| R-factor | 0.1855 |
| Rwork | 0.185 |
| R-free | 0.21180 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.363 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.077 | 0.396 |
| Number of reflections | 20248 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 95.2 | 67 |
| Redundancy | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 289 | lithium chloride, Bis-tris buffer, glycerol, isopropanol, PEG 3350, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
