1R0O
Crystal Structure of the Heterodimeric Ecdysone Receptor DNA-binding Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.2834 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.370, 59.880, 113.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.240 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.26400 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r0n |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.088 | 0.456 |
Number of reflections | 15928 * | |
<I/σ(I)> | 22 | 5.8 |
Completeness [%] | 99.6 * | 97.8 |
Redundancy | 7.5 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 8 * | Zhao, Q., (2000) J.Mol.Biol., 296, 509. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
10 | 1 | 2 | MES | ||
11 | 1 | 2 | H2O | ||
2 | 1 | 1 | Sodium Chloride | ||
3 | 1 | 1 | DTT | ||
4 | 1 | 1 | Magnesium Chloride | ||
5 | 1 | 1 | MES | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG 3350 | ||
8 | 1 | 2 | DTT | ||
9 | 1 | 2 | Magnesium Chloride |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 25 (mM) | |
2 | 1 | drop | protein | 0.9 (mM) | |
3 | 1 | drop | DNA | 0.45 (mM) | |
4 | 1 | reservoir | PEG3350 | 18-23 (%) | |
5 | 1 | reservoir | Tris | 25 (mM) | |
6 | 1 | reservoir | 5 (mM) | ||
7 | 1 | reservoir | 400 (mM) |