1R0D
HIP1R THATCH DOMAIN CORE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-12-06 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0332, 0.97967, 0.97954, 0.96430 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.200, 165.900, 109.800 |
| Unit cell angles | 90.00, 132.40, 90.00 |
Refinement procedure
| Resolution | 19.880 - 1.900 |
| R-factor | 0.2241 |
| Rwork | 0.224 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.433 |
| Number of reflections | 147170 | |
| <I/σ(I)> | 23.5 | 2.4 |
| Completeness [%] | 95.7 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 1.75 M ammonium sulfate, 2.5% ethylene glycol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 1.75 M ammonium sulfate, 2.5% ethylene glycol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
