1QZX
Crystal structure of the complete core of archaeal SRP and implications for inter-domain communication
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9740 |
Spacegroup name | I 4 |
Unit cell lengths | 197.906, 197.906, 64.305 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 4.000 |
Rwork | 0.313 |
R-free | 0.38300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j8m |
RMSD bond length | 0.016 |
RMSD bond angle | 1.930 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | BEAST |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 4.220 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.083 * | 0.484 * |
Number of reflections | 9576 * | |
<I/σ(I)> | 4.8 | 1.5 |
Completeness [%] | 89.0 * | 97.9 * |
Redundancy | 4.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Rosendal, K.R., (2004) Acta Crystallogr.,Sect.D, 60, 140. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 2-4 (%) | |
3 | 1 | reservoir | 1-1.2 (M) |