1QZW
Crystal structure of the complete core of archaeal SRP and implications for inter-domain communication
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 31 |
| Unit cell lengths | 137.763, 137.763, 307.894 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 4.100 |
| Rwork | 0.340 |
| R-free | 0.38700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1J8M |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.260 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.320 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.087 * | 0.481 * |
| Number of reflections | 49660 * | |
| <I/σ(I)> | 4 | 1.5 |
| Completeness [%] | 97.2 * | 98.1 * |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | Rosendal, K.R., (2004) Acta Crystallogr.,Sect.D, 60, 140. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium sulphate | ||
| 2 | 1 | 1 | sodium chloride | ||
| 3 | 1 | 1 | sodium acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Ammonium sulphate | ||
| 6 | 1 | 2 | sodium acetate | ||
| 7 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.26 (M) | pH4.5 |
| 3 | 1 | reservoir | 200 (mM) |
