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1QZT

Phosphotransacetylase from Methanosarcina thermophila

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X26C
Synchrotron siteNSLS
BeamlineX26C
Temperature [K]100
Detector technologyCCD
Collection date2003-06-01
DetectorADSC QUANTUM 4
Wavelength(s)1.1
Spacegroup nameP 41
Unit cell lengths115.345, 115.345, 129.366
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.700
R-factor0.24864
Rwork0.247
R-free0.28636
RMSD bond length0.016
RMSD bond angle1.549
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareMLPHARE
Refinement softwareREFMAC (5.1.24)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.690
High resolution limit [Å]2.6002.600
Rmerge0.084
Number of reflections49910
Completeness [%]96.897.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5294sodium acetate, ammonium sulfate, DTT, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K

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