1QZH
Crystal structure of Pot1 (protection of telomere)- ssDNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2003-02-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0688 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.614, 76.329, 140.130 |
| Unit cell angles | 90.00, 115.21, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.430 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.062 * | |
| Total number of observations | 132615 * | |
| Number of reflections | 43793 | |
| Completeness [%] | 89.4 * | 78.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 16 * | PEG4000, TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | TRIS | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | PEG4000 | ||
| 5 | 1 | 2 | TRIS | ||
| 6 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
| 2 | 1 | reservoir | PEG4000 | 27-30 (%) | |
| 3 | 1 | reservoir | dithiothreitol | 5 (mM) |
