1QZ4
Structure of YcfC Protein of Unknown Function Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-24 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.999871 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.102, 62.749, 68.399 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.15729 |
| Structure solution method | MIRAS |
| Starting model (for MR) | Build by WARP/ARP |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.582 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.107 | 0.423 |
| Number of reflections | 11477 | |
| <I/σ(I)> | 15.87 | 1.8 |
| Completeness [%] | 82.6 | 36.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, zinc acetate, lithium acetate , VAPOR DIFFUSION, HANGING DROP, temperature 293K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, zinc acetate, lithium acetate , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
