1QYY
Crystal Structure of N-Terminal Domain of Human Platelet Receptor Glycoprotein Ib-alpha at 2.8 Angstrom Resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.071564, 1.0079675 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.610, 113.820, 56.210 |
| Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.223 |
| Rwork | 0.219 |
| R-free | 0.28210 * |
| Structure solution method | MAD |
| Starting model (for MR) | Ab initio |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 * | 0.317 * |
| Total number of observations | 118489 * | |
| Number of reflections | 16932 | 1889 * |
| <I/σ(I)> | 8.7 | 2.2 |
| Completeness [%] | 94.6 | 72.2 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | PEG 6000, SODIUM NITRATE, SODIUM ACETATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 12 (%(w/w)) | |
| 2 | 1 | reservoir | sodium acetate | 120 (mM) | |
| 3 | 1 | reservoir | sodium nitrate | 200 (mM) | pH5.6 |
