1QYU
Structure of the catalytic domain of 23S rRNA pseudouridine synthase RluD
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2002-09-07 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.978795, 0.978462, 0.950037 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.140, 75.140, 181.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.000 |
| Rwork | 0.219 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.133 |
| Number of reflections | 36112 | |
| <I/σ(I)> | 38.3 | 9.2 |
| Completeness [%] | 95.1 | 69.6 |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Del Campo, M., (2003) Acta Cryst., D59, 1871. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ethylene glycol | 25-40 (%) |
