1QYN

Crystal Structure of SecB from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-03-08
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	84.005, 84.005, 204.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 * - 2.350
R-factor	0.234
Rwork	0.234
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fx3
RMSD bond length	0.011
RMSD bond angle	1.360 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.610	2.500
High resolution limit [Å]	2.350	2.350
Rmerge	0.045 *	0.219
Total number of observations	465596 *
Number of reflections	33605 *
<I/σ(I)>	28.6	5
Completeness [%]	95.8 *	96.7
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	Dekker, C., (2003) J.Struct.Biol., 144, 313. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		80 (mM)
2	1	reservoir	MPD	28 (%(v/v))
3	1	drop	protein	10 (mg/ml)