1QYM

X-ray structure of human gankyrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-05-19
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	116.373, 116.373, 74.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.187
R-free	0.23300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SWISS-MODEL (PDB ENTRIES 1N1I 1n0r 1mj0 1N0Q)
RMSD bond length	0.011
RMSD bond angle	1.281
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.072	0.620
Total number of observations	169487 *
Number of reflections	12978
<I/σ(I)>	35.4	4
Completeness [%]	99.8	99.3
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	20 *	3.5M Na Formate , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	18.3 (mg/ml)
2	1	reservoir	sodium formate	3.5 (M)	pH7.0