1QYM
X-ray structure of human gankyrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-05-19 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 116.373, 116.373, 74.399 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
Rwork | 0.187 |
R-free | 0.23300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SWISS-MODEL (PDB ENTRIES 1N1I 1n0r 1mj0 1N0Q) |
RMSD bond length | 0.011 |
RMSD bond angle | 1.281 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.072 | 0.620 |
Total number of observations | 169487 * | |
Number of reflections | 12978 | |
<I/σ(I)> | 35.4 | 4 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 20 * | 3.5M Na Formate , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18.3 (mg/ml) | |
2 | 1 | reservoir | sodium formate | 3.5 (M) | pH7.0 |