1QYL
GCATGCT + Vanadium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8416 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 22.532, 58.469, 24.148 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.800 - 1.000 |
| R-factor | 0.1493 |
| Rwork | 0.149 |
| R-free | 0.18380 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | NDB entry udg028 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 0.042 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 5.800 | 1.200 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.095 | 0.147 |
| Number of reflections | 8848 | |
| <I/σ(I)> | 20.1 | 12.63 |
| Completeness [%] | 98.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 290 | NaCacodylate, Vanadium Chloride, Sodium Chloride, MPD, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaCacodylate | ||
| 2 | 1 | 1 | Vanadium Chloride | ||
| 3 | 1 | 1 | Sodium Chloride | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | NaCacodylate | ||
| 7 | 1 | 2 | Vanadium Chloride | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | H2O |
