1QYI
X-RAY STRUCTURE OF Q8NW41 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ZR25.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97942, 0.97922, 0.97236 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 87.480, 87.480, 115.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.500 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.27800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.062 | 0.146 |
Number of reflections | 23940 | |
Completeness [%] | 89.5 | 71.6 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% PEG 3350, 0.2M Lithium nitrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |