Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1QY8

Crystal Structure of the N-domain of the ER Hsp90 chaperone GRP94 in complex with Radicicol

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-BM
Synchrotron siteAPS
Beamline19-BM
Temperature [K]100
Detector technologyCCD
Collection date2001-09-14
DetectorCUSTOM-MADE
Wavelength(s)1.0332
Spacegroup nameC 2 2 21
Unit cell lengths86.749, 99.367, 63.298
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution6.000

*

- 1.850
Rwork0.222
R-free0.27700

*

Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1QY5
RMSD bond length0.011
RMSD bond angle1.470

*

Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.920
High resolution limit [Å]1.8501.850
Rmerge0.055

*

0.440
Number of reflections23549
<I/σ(I)>36.31.89
Completeness [%]99.095.5
Redundancy7.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.618

*

PEG 550 MME, Magnesium chloride, Tris, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11drop10-50 (mM)
21dropprotein30 (mg/ml)
31reservoirTris100 (mM)pH7.6
41reservoir200-300 (mM)
51reservoirPEG40035-45 (%)

217705

PDB entries from 2024-03-27

PDB statisticsPDBj update infoContact PDBjnumon