1QY6

Structue of V8 Protease from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	300
Detector technology	DIFFRACTOMETER
Collection date	1998-02-24
Detector	WEISSENBERG
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	62.721, 62.721, 225.876
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.23
Rwork	0.200
R-free	0.23000
Structure solution method	MAD
RMSD bond length	0.010 *
RMSD bond angle	1.380
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.090	0.410 *
Total number of observations	97623 *
Number of reflections	20095	790 *
Completeness [%]	92.0	76.2 *
Redundancy	6.3 *	4.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	293	PEG 5000 mme, KCl, HEPES, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	HEPES	50 (mM)	pH8.6
3	1	drop		50 (mM)
4	1	drop	PEG5000 MME	15 (%)
5	1	reservoir		100 (mM)
6	1	reservoir	HEPES	100 (mM)
7	1	reservoir	PEG5000 MME	20 (%)