1QXZ
Crystal structure of S. aureus methionine aminopeptidase in complex with a ketoheterocycle inhibitor 119
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 2001-05-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.57 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.947, 76.916, 41.651 |
Unit cell angles | 90.00, 104.79, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.680 |
R-factor | 0.17122 |
Rwork | 0.170 |
R-free | 0.20000 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 * |
RMSD bond angle | 0.847 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.770 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.041 * | 0.143 |
Number of reflections | 28461 | |
<I/σ(I)> | 22.2 | 6.3 |
Completeness [%] | 97.9 | 93.1 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7.5 * | 294 | Oefner, C., (2003) J. Mol. Biol., 332, 13. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 12 (mg/ml) | |
2 | 1 | 1 | HEPES | 50 (mM) | pH7.5 |
3 | 1 | 1 | 150 (mM) | ||
4 | 1 | 1 | glycerol | 5 (%) | |
5 | 1 | 1 | TCEP | 1 (mM) |