1QX0
CONJUGATIVE RELAXASE TRWC IN COMPLEX WITH ORIT DNA. METAL-BOUND STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-12-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.185, 91.185, 205.034 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.000 - 2.260 |
| R-factor | 0.239 |
| Rwork | 0.234 |
| R-free | 0.28100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1omh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.497 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.115 | 0.471 |
| Number of reflections | 24256 | |
| <I/σ(I)> | 3.6 | 1.4 |
| Completeness [%] | 99.3 | 96.7 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 4 * | pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 MME | 25 (%(w/v)) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
