1QWY
Latent LytM at 1.3 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Wavelength(s) | 1.28335 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.332, 53.232, 51.603 |
Unit cell angles | 90.00, 104.02, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.300 |
R-factor | 0.16362 |
Rwork | 0.163 |
R-free | 0.18100 * |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.370 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.062 | 0.174 |
Number of reflections | 54733 | |
<I/σ(I)> | 6.4 | 3.5 |
Completeness [%] | 94.0 | 91.1 |
Redundancy | 2.3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 21 * | 170 mM ammonium sulfate, 25.5% (w/v) PEG 8K, 15% (v/v) glycerol, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 21 * | 170 mM ammonium sulfate, 25.5% (w/v) PEG 8K, 15% (v/v) glycerol, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | Tris | 5 (mM) | pH7.5 |
3 | 1 | reservoir | ammonium sulfate | 170 (mM) | |
4 | 1 | reservoir | PEG8000 | 25.5 (%(w/v)) | |
5 | 1 | reservoir | glycerol | 15 (%(v/v)) |