1QWY

Latent LytM at 1.3 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Wavelength(s)	1.28335
Spacegroup name	P 1 21 1
Unit cell lengths	45.332, 53.232, 51.603
Unit cell angles	90.00, 104.02, 90.00

Refinement procedure

Resolution	15.000 - 1.300
R-factor	0.16362
Rwork	0.163
R-free	0.18100 *
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.370
High resolution limit [Å]	1.300	1.300
Rmerge	0.062	0.174
Number of reflections	54733
<I/σ(I)>	6.4	3.5
Completeness [%]	94.0	91.1
Redundancy	2.3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	21 *	170 mM ammonium sulfate, 25.5% (w/v) PEG 8K, 15% (v/v) glycerol, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	21 *	170 mM ammonium sulfate, 25.5% (w/v) PEG 8K, 15% (v/v) glycerol, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	Tris	5 (mM)	pH7.5
3	1	reservoir	ammonium sulfate	170 (mM)
4	1	reservoir	PEG8000	25.5 (%(w/v))
5	1	reservoir	glycerol	15 (%(v/v))