1QWL
Structure of Helicobacter pylori catalase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.264, 154.502, 95.762 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.600 |
| R-factor | 0.18331 |
| Rwork | 0.181 |
| R-free | 0.21900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Human erythrocyte catalase |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 * | 0.137 * |
| Number of reflections | 119258 * | 7699 * |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 94.4 | 87.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 15% PEG MME 550, 0.1 M sodium citrate, 10 mM ZnSO4, 3 mM NaN3, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | PEG550 MME | 15 (%) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | 3 (mM) |
