1QWI
Crystal Structure of E. coli OsmC
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9795, 0.9793, 0.9770 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.747, 64.229, 67.585 |
Unit cell angles | 90.00, 92.62, 90.00 |
Refinement procedure
Resolution | 500.000 * - 1.800 |
Rwork | 0.222 |
R-free | 0.24000 * |
Structure solution method | MAD |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.360 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.880 |
High resolution limit [Å] | 1.800 | 2.000 * |
Rmerge | 0.039 * | |
Number of reflections | 48947 | |
Completeness [%] | 99.7 * | 98.7 * |
Redundancy | 2.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 5000 MME, 0.2M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris | 5 (mM) | pH8.0 |
3 | 1 | drop | dithiothreitol | 5 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
5 | 1 | reservoir | PEG5000 MME | 30 (%) | |
6 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |