1QW0
Crystal Structure of Haemophilus influenzae N175L mutant Holo Ferric ion-Binding Protein A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-05 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.850, 76.037, 34.067 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.000 * - 1.900 |
| R-factor | 0.22214 |
| Rwork | 0.220 |
| R-free | 0.27100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d9v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.087 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 * | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 * | 0.168 * |
| Number of reflections | 20739 | |
| Completeness [%] | 97.2 * | 90.8 |
| Redundancy | 5.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 4 * | PEG 550 MME, Tris, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | PEG550 MME | 18 (%) | |
| 3 | 1 | drop | Tris | 0.05 (M) | pH8.2 |
| 4 | 1 | reservoir | PEG550 MME | 36 (%) | |
| 5 | 1 | reservoir | Tris | 0.1 (M) | pH8.2 |
