1QVJ
structure of NUDT9 complexed with ribose-5-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2002-09-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.900, 87.455, 120.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.900* |
| Rwork | 0.212 |
| R-free | 0.24300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q33 |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.233 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 1.980 |
| High resolution limit [Å] | 1.900 * | 1.910 |
| Rmerge | 0.043 * | 0.311 * |
| Total number of observations | 171127 * | |
| Number of reflections | 31006 * | |
| <I/σ(I)> | 35.4 | 4.4 |
| Completeness [%] | 94.8 | 88.9 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 298 | Ammonium sulfate, magnisum chloride, trisHCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 25 (mM) | pH8.5 |
| 3 | 1 | reservoir | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | 20 (mM) | ||
| 6 | 1 | reservoir | 150 (mM) |
