1QVF
Structure of a deacylated tRNA minihelix bound to the E site of the large ribosomal subunit of Haloarcula marismortui
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Detector technology | CCD |
| Collection date | 2003-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0015 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 213.522, 300.817, 574.861 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| Rwork | 0.196 |
| R-free | 0.25800 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jj2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Total number of observations | 1662618 * | |
| Number of reflections | 327612 | |
| <I/σ(I)> | 13.45 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | back-extraction, vapor diffusion * | 7.1 * | Ban, N., (2000) Science, 289, 905. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.2 (M) | ||
| 2 | 1 | reservoir | 0.5 (M) | ||
| 3 | 1 | reservoir | Kacetate | 100 (mM) | |
| 4 | 1 | reservoir | 30 (mM) | ||
| 5 | 1 | reservoir | PEG6000 | 7 (%) | |
| 6 | 1 | reservoir | Tris | 15 (mM) | |
| 7 | 1 | reservoir | MES | 15 (mM) | |
| 8 | 1 | reservoir | 1 (mM) |
