1QVE
Crystal structure of the truncated K122-4 pilin from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 |
Unit cell lengths | 40.184, 38.930, 37.228 |
Unit cell angles | 66.39, 111.11, 93.74 |
Refinement procedure
Resolution | 35.580 - 1.540 |
R-factor | 0.14112 |
Rwork | 0.139 |
R-free | 0.17855 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ayz |
RMSD bond length | 0.014 |
RMSD bond angle | 1.468 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 1.610 |
High resolution limit [Å] | 1.540 | 1.540 |
Number of reflections | 26457 | |
<I/σ(I)> | 16.5 | 6.6 |
Completeness [%] | 93.1 | 88.5 |
Redundancy | 2.7 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 4000, Potassium Phosphate Monobasic, Sodium Cacodylate, Tris HCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |