1QVC

CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	288
Detector technology	CCD
Collection date	1997-01-19
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	105.369, 62.923, 97.792
Unit cell angles	90.00, 112.57, 90.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.247
Rwork	0.247
R-free	0.31700
RMSD bond length	0.006
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.000	2.370
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.197
Total number of observations	150790 *
Number of reflections	24956
<I/σ(I)>	15.8
Completeness [%]	82.7	43.5
Redundancy	6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	9	4, 20 *	SODIUM CHLORIDE, AMMONIUM SULFATE, pH 9.0, SMALL TUBES, temperature 288K
1	Batch method *	9	4, 20 *	SODIUM CHLORIDE, AMMONIUM SULFATE, pH 9.0, SMALL TUBES, temperature 288K
1	Batch method *	9	4, 20 *	SODIUM CHLORIDE, AMMONIUM SULFATE, pH 9.0, SMALL TUBES, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	14 (mg/ml)
2	1	1	Tris-HCl	50 (mM)
3	1	1		0.325 (M)
4	1	1	ammonium sulfate	0.3-0.6 (M)