1QUQ
COMPLEX OF REPLICATION PROTEIN A SUBUNITS RPA14 AND RPA32
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-01 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.700, 76.600, 119.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.29100 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.044 | 0.162 * |
| Total number of observations | 123441 * | |
| Number of reflections | 23882 | |
| <I/σ(I)> | 34.4 | |
| Completeness [%] | 98.6 | 96.9 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 0.1 M MES, 0.75 M AMMONIUM SULPHATE, 20% PEG 8K, 10 MM DTT., pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 0.1 (M) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.75 (M) | |
| 3 | 1 | reservoir | PEG8000 | 20 (%) | |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | protain | 15 (mg/ml) | |
| 6 | 1 | drop | HEPES | 1 (mM) | |
| 7 | 1 | drop | 50 (mM) | ||
| 8 | 1 | drop | dithiothreitol | 10 (mM) |
