1QUM
CRYSTAL STRUCTURE OF ESCHERICHIA COLI ENDONUCLEASE IV IN COMPLEX WITH DAMAGED DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-05 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.120, 58.727, 51.146 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.093 * |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.048 | 0.328 |
| Total number of observations | 150389 * | |
| Number of reflections | 53884 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 89.0 | 70.3 |
| Redundancy | 2.8 | 1.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | MPEG 2000, MES BUFFER, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES BUFFER | ||
| 2 | 1 | 1 | MPEG 2000 | ||
| 3 | 1 | 2 | MPEG 2000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | mPEG2000 | 20 (%) | |
| 2 | 1 | reservoir | MES | 100 (mM) |
