1QUI
PHOSPHATE-BINDING PROTEIN MUTANT WITH ASP 137 REPLACED BY GLY COMPLEX WITH BROMINE AND PHOSPHATE
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.970, 63.980, 123.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.21800 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.790 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Rmerge | 0.047 |
Number of reflections | 25234 |
Completeness [%] | 90.0 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 * | Wang, Z., (1994) J.Biol.Chem., 269, 25091. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.25 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 1.5 (mM) | |
3 | 1 | drop | PEG6000 | 13.5 (%(w/v)) | |
4 | 1 | drop | 37.5 (mM) | ||
5 | 1 | drop | potassium acetate | 15 (mM) | |
6 | 1 | reservoir | PEG6000 | 18 (%(w/v)) | |
7 | 1 | reservoir | 50 (mM) | ||
8 | 1 | reservoir | potassium phosphate | 2 (mM) | |
9 | 1 | reservoir | potassium acetate | 20 (mM) |