1QUH

L99G/E108V MUTANT OF T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	AREA DETECTOR
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 32 2 1
Unit cell lengths	61.000, 61.000, 97.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.000 - 1.850
R-factor	0.181
RMSD bond length	0.017
RMSD bond angle	2.800
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.990
High resolution limit [Å]	1.850	1.850
Rmerge	0.073	0.237
Number of reflections	17587 *
<I/σ(I)>	6.7
Completeness [%]	91.0 *	91
Redundancy	2.5	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphate	2.0 (M)
3	1	reservoir		0.25 (M)
4	1	reservoir	oxidized beta-mercaptoethanol