1QUF
X-RAY STRUCTURE OF A COMPLEX NADP+-FERREDOXIN:NADP+ REDUCTASE FROM THE CYANOBACTERIUM ANABAENA PCC 7119 AT 2.25 ANGSTROMS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-05 |
| Detector | SIEMENS |
| Spacegroup name | P 65 |
| Unit cell lengths | 85.840, 85.840, 97.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.250 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ANABAENA NATIVE FNR X-RAY MODEL AT 1.8 ANGSTROMS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.450 |
| Data reduction software | XDS |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.073 * | |
| Total number of observations | 48735 * | |
| Number of reflections | 15544 | |
| Completeness [%] | 83.0 | 34 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | SEE REFERENCE 1., pH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.6 (mg/ml) | |
| 2 | 1 | drop | beta-octylglucoside | 1 (%(w/v)) | |
| 3 | 1 | drop | PEG6000 | 8 (%(w/v)) | |
| 4 | 1 | drop | sodium azide | 0.04 (%(w/v)) | |
| 5 | 1 | drop | Mes-NAOH | 0.04 (M) | |
| 6 | 1 | reservoir | Mes-NAOH | 0.1 (M) | |
| 7 | 1 | reservoir | PEG6000 | 20 (%(w/v)) | |
| 8 | 1 | reservoir | sodium azide | 0.1 (%(w/v)) |
