1QUE
X-RAY STRUCTURE OF THE FERREDOXIN:NADP+ REDUCTASE FROM THE CYANOBACTERIUM ANABAENA PCC 7119 AT 1.8 ANGSTROMS
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 300 |
Detector technology | AREA DETECTOR |
Collection date | 1992-10 |
Detector | SIEMENS |
Spacegroup name | P 65 |
Unit cell lengths | 88.200, 88.200, 97.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.800 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.21400 |
Structure solution method | MIR |
RMSD bond length | 0.030 |
RMSD bond angle | 2.500 |
Data reduction software | XDS |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | PHASES |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 * | |
Total number of observations | 96286 * | |
Number of reflections | 20337 | |
Completeness [%] | 85.0 | 34 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | SEE REFERENCE 1., pH 7.0, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10.6 (mg/ml) | |
10 | 1 | reservoir | sodium azide | 0.1 (%(w/v)) | |
11 | 1 | reservoir | MES-NaOH | 0.1 (M) | |
2 | 1 | drop | Tris-HCl | 4 (mM) | |
3 | 1 | drop | beta-OG | 1 (%(w/v)) | |
4 | 1 | drop | PEG6000 | 8 (%(w/v)) | |
5 | 1 | drop | ammonium sulfate | 8 (mM) | |
6 | 1 | drop | sodium azide | 0.04 (%(w/v)) | |
7 | 1 | drop | MES-NaOH | 0.04 (M) | |
8 | 1 | reservoir | PEG6000 | 20 (%(w/v)) | |
9 | 1 | reservoir | ammonium sulfate | 20 (mM) |