1QU7
FOUR HELICAL-BUNDLE STRUCTURE OF THE CYTOPLASMIC DOMAIN OF A SERINE CHEMOTAXIS RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 132.440, 132.440, 134.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 2.600 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.27600 |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.510 * |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.125 | 0.745 |
| Number of reflections | 40467 | |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 95.6 | 74 |
| Redundancy | 3.84 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sparse matrix method * | 6.5 | 4 * | MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | EDTA | 1 (mM) | |
| 2 | 1 | 1 | HEPES | 25 (mM) | pH7.4 |
| 3 | 1 | 1 | 5-10 (mg/ml) | ||
| 4 | 1 | 2 | N-[2-acetamide]-2-iminodiacetic acid | 0.1 (M) | pH6.5 |
| 5 | 1 | 2 | MPD | 15-20 (%) | |
| 6 | 1 | 2 | sodium citrate | 0.2 (M) |
